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Aptamer-based sensor with high-specificity typically has a fixed linear range due to the host-guest binding. To expand the dynamic range for multiple scenarios, we report here a dual-ratiometric electrochemical aptasensor that integrates two aptamer profiles with varied affinity into a single sensing interface for aflatoxin B1 (AFB1) detection. Using functional aptamer as recognition element and generator of signals, we fabricate the dual-ratiometric aptasensor with anthraquinone loaded reduced graphene oxide (AQ-rGO), methylene blue l