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Sub-Saharan region in Africa still holds the highest burden of HIV/AIDS globally. HIV-1 requires coreceptor to gain entry into permissive cells to initiate infection. Molecular analysis of the chemokine coreceptor usage is important clinically and in the effective management of AIDS virus. This study aims to determine the coreceptor usage among HIV-1 drug naïve patients residing in the Eastern Cape, South Africa. We collected blood samples from 55 HIV infected patients into anticoagulant vacutainer. RNA was extracted from seperated plasm