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The enzyme is really characterized in plants and creatures and crystal frameworks of β-amylases are obtained from sweet-potato, soybean and Bacillus cereus. OBJECTIVE Find out correlation between structural and useful security caused by changing in pH, heat and chaotropes. METHODS Activity, intrinsic fluorescence, extrinsic fluorescence, near- and far- ultraviolet circular dichroism spectroscopic measurements were done. RESULTS Peaks about 208 nm and 222 nm obtained