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It also resulted in considerable, albeit less radical, fluorescence decrease at one day postfertilization in Tg(myl7GFP) and Tg(fli1EGFP) fish that express EGFP zygotically. StCsm targeted against the endogenous tdgf1 elicited the characteristic one-eyed phenotype with higher than 50% penetrance, and therefore with comparable effectiveness to morpholino-mediated knockdown. We conclude that Csm-mediated knockdown is quite efficient for maternal tr
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