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Due to the aggregation of AgNPs, a simple colorimetric approach was also studied for quantitation of betahistine in the range 1.0-20 μM. The limit of detection for fluorescence measurement and colorimetric approach was 0.02 μM and 0.23 μM, respectively. Further, the proposed method exhibited excellent selectivity towards betahistine in presence of several cations, biomolecules such as glucose, uric acid, creatinine, amino acids and several anti-vertigo medications. The method was applied to quantify betahistine from pharmaceutical products and results obtai