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All strains belonged to the Mycobacterium bovis species. A two-step analysis of genetic affinity allowed 50 strains to be identified as phylogenetically closely related and separated between three genetic clusters consisting of 2 to 27 strains. Conclusion Based on the results of genotyping, bTB outbreaks were found in three herds, and three transmission chains were identified among these herds. © 2020 M. Krajewska-Wędzina et al. published by Sciendo.Introduction Sheep pulmonary adenomatosis (ovine pulmonary adenomatosis, OPA, Jaagsi