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To a smaller level, this might be additionally observed for the much stronger bidentate SAv-Bt complex. This shows the necessity of a careful and quantitative evaluation of tip effects into the HS-AFM imaging additionally of highly binding methods. The displayed DNA origami-based assay are universally utilized to quantify tip effects in highly and weakly binding methods and also to enhance the experimental options due to their dependable HS-AFM imaging.Glioblastoma is co