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The GOS intervention mainly affected the lipid and amino acid metabolism of the piglets. Meanwhile, the mRNA levels of CPT-1 and PCG1α increased in the GOS piglets' liver. Additionally, with the accumulation of antioxidant-related metabolites in the GOS piglet liver, the hepatic mitochondrial antioxidant capacity increased, and the phosphorylation of AMP-activated protein kinase and the protein expression levels of nuclear factor-erythroid-2-related factor 2 and heme oxygenase-1 were also upregulated in the liver. In conclusion, our findi