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alic acid binding site on HN that we propose to be the site involved in activating F. We use cryo-electron tomography of authentic intact viral particles for the first time to directly assess the mechanism of action of this treatment on the conformation of the viral F protein and present the first direct observation of the induced conformational rearrangement in the viral F protein. Copyright © 2020 Marcink et al.Poor penetration through the outer membrane (OM) of Gram-negative bacteria is a major barrier of antibiotic develop