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GST pulldown and mass spectrometry had been done to analyze the interacting protein of TIMP2 and immunofluorescence test ended up being used to look for the location of the protein in HK-2 cells. Cell viability along with the appearance standard of CCND1, C-FOS, MAPK1 and P-MAPK1 were detected into the examples treated by overexpressed TIMP2 as well as the inhibitor for the interacting protein KIT. TIMP2 substantially inhibited cell proliferation compared to the control and BB-94-treated gr