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This study investigated nitrification process during cattle manure-maize straw (CM) and biochar (CM composting in terms of multi-variable interaction (MVI) among environmental parameters, ammonia-oxidizing archaea (AOA) and bacteria (AO community structure, nitrogen-related enzymes as well as substrates using structural equation model (SEM). Results showed that adding biochar significantly reduced potential ammonia oxidation rates. SEM analysis revealed that AOB was affected by temperature and pH, which stimulated the release of ureas