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pH cycling was observed. The surface loss was small compared with the overall lesion depth and thickness of the generated acid-resistant layer. Lasers Surg. Med. © 2020 Wiley Periodicals LLC.Cholangiocarcinoma (CCA) is a rare tumor which requires a multimodality approach for its diagnosis. Carbohydrate antigen 19-9 (CA19-9) is currently the most commonly used tumor marker for CCA; nevertheless, it has certain limitations which need to be considered when using it as a tumor marker. MiRNA-150 altered expression has been linked to the develo