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No samples resulted positive for Flavivirus by RT-PCR assay. CONCLUSION Although a moderately high percentage of animals with specific antibody for WNV has been detected in wild boar in other surveillance studies in Europe, this has not been reported previously in Italy. Together, these data indicate that European wild boar are exposed to WNV and/or other related-Flavivirus in central Italy and confirm the usefulness of wild ungulates, as suitable Flavivirus sentinels.Rheumatoid Arthritis (RA), Systemic lupus erythematosus (SLE) and an