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The principle is beneficial for the mathematical analyses of complex biochemical reactions involving two or more kinds of enzymes, for those involving two or more substrates or for those involving both.We present a label-free liquid crystal-based biosensor for the detection of dopamine (DA) in aqueous solutions using dopamine-binding aptamers (DBA) as recognition elements. In this system, the dimethyloctadecyl [3-(trimethoxysilyl) propyl] ammonium chloride (DMOAP) self-assembled monolayers immobilized on glass slides support the long alkyl chains that keep