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Additionally, to evaluate bioactivity, primary human gingival fibroblasts (hGFs) were cultured onto various TNPs in vitro. The findings confirmed that the proliferation and morphology of hGFs were enhanced on 10-20 h aged electrolyte anodized TNPs. This pioneering study systematically investigates the optimization of anodization electrolyte toward fabricating nanoporous implants with desirable characteristics.With the urgent market demand for high-energy-density batteries, the alloy-type or conversion-type anodes with high specific capa