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Outcomes showed 7 nm magnetite nanoparticles loaded into chitosan/Tween 80 microspheres with normal sizes of 1.431 µm. At concentrations from 1 to 100 µg/mL, there is no proof alterations in mitochondrial metabolic process, cellular morphology, membrane rupture, cell pattern, nor sister chromatid change development. For each microgram of microspheres 1.8 µg of BSA was immobilized. The end result supplies the fundamental comprehension of the inside vitro biological behavior, and safety, of developed