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As a result, this process managed to quantify DA from 1 to 300 nM with a detection limitation of 0.74 nM, which exhibited a higher specificity against cerebral interferents. Additionally, the practicability of this platform was successfully demonstrated through dedication of the dynamics of cerebral DA into the activities of high K+ and nomifensine retromicrodialysis. Graphical abstract.In this study, both traditional one-dimensional liquid chromatography (1DLC) and comprehensive two-dimension