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The hCG-H levels when you look at the culture news were examined by ELISA kit (Cusabio Biotech, CBS-E15803h) according to the maker's directions. Analytical analysis ended up being performed making use of SPSS v.21 (IBM Corp., Armonk, NY, USA). RESULTS The NGS analysis revealed that 36% regarding the embryos (n = 56) were balanced, and 64% regarding the embryos had been unbalanced (n = 99). The existence of hCG-H was verified in all embryo culture media examples but had been missing into the bad control