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We demonstrate a sequential electrospinning process involving the adsorption of ZnO nanoparticles on the surface of bio-based polyester, which is a terpolyester of a renewable isosorbide (IS monomer, ethylene glycol, 1,4-cyclohexane dimethanol, and terephthalic acid, the-so-called PEICT, to fabricate stable ZnO nanoparticles/PEICT nanofiber composite system protected with other two PEICT nanofiber mats. We found that post-electrospinning treatment with a particular solvent was effective to remove a residual solvent molecule in the PE