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Different materials have been used to repair root perforations, the most successful of which is mineral trioxide aggregate (MTA). It is technically difficult to use MTA for perforation repair. Recently, some bio-ceramic sealers such as Endoseal MTA were introduced to repair the perforation site during root filling, which decreases the technical difficulty of this procedure. The aim of this study was to compare the bacterial microleakage of Pro-Root MTA and Endoseal MTA sealer in root perforation repair. This experimental study evaluated