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9 × 10 CFU/mL among the experimental herbal solutions groups. has shown maximum bacterial count followed by and . NaOCl 5% showed maximum antibacterial activity against 3-week biofilm on tooth substrate. , , and showed statistically significant antibacterial activity against 3-week biofilm. The use of herbal alternatives might prove to be advantageous considering the several undesirable characteristics of NaOCl. NaOCl 5% showed maximum antibacterial activity against 3-week biofilm on tooth substrate. T. indica, P. amarus, and C. longa s