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05) but increased in PF animals (P less then 0.05) from period 1 to period 2. There was a feed intake effect on BHD1 expression (P = 0.04) and a tendency for a thermal environment effect (P = 0.08), with expression increasing in both cases. Expression of MCT4 was affected by feed intake (P = 0.003) as were all NHE genes (NHE1, NHE2, and NHE3; P less then 0.05). These results indicate that with low feed intake and heat stress, there are shifts in rumen VFA dynamics and in the capacity of the rumen epithelium to absorb and transport V