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This study aimed to analyze the chemical structure of C. tiglium gas (CTEO) extracted from the seeds of C. tiglium as well as its cytotoxicity and antitumor impact in vitro. Supercritical CO2 substance extraction technology was made use of to extract CTEO and the chemical constituents of the essential oil had been identified by evaluating the retention indices and size spectra information extracted from the NIST collection with those computed in line with the C7-C40 n-alkanes standard. In vitro cytotoxicity