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Using a whole transcriptome correlation, a 3-gene panel, including ESRP2, PTK6, and MAGEH1, is designated to assess PDAC tumor sample cellularity by PCR. One MUC4 SV (MUC4Δ6) and one MUC13 SV (MUC13WT) are quantified in a separate PDAC patient cohort, and their effects on survival are experimentally validated. Altogether, we demonstrate the unique expression pattern of mucins, four mucins-based PDAC subtypes, and the contribution of MUC1, MUC4, and MUC16 SVs in PDAC patient survival. Altogether, we