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The inter-modality correlation of the thickness values was good (ICC 0.76 [0.626-0.846]), indicating that 3D analysis of epithelial thickness provides valid results. Intra-rater and inter-rater reliability were good (3D analysis) and excellent (2D analysis), suggesting high reproducibility. Diagnostic accuracy was high for the developed 3D analysis of oral epithelia using non-invasive, radiation-free OCT imaging. This new 3D technique could potentially be used to improve time-efficiency and quality in the diagnosis of epithelial lesions
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