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Bacillus laterosporus Bl13 has good antagonistic effect on the pathogen of tomato early blight (TE disease. A pot experiment was conducted to investigate the effect and mechanism of B. laterosporus Bl13 against TEB disease by measuring biological traits such as plant height, stem diameter, disease index of TEB, activity of plant defense enzyme in leaves, and microbial diversity and community composition in root area. The results showed that B. laterosporus Bl13 could significantly reduce the disease index of TEB disease incidence, inc