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Also, both serum and cultured viruses could be sequenced as a collection of 25 overlapping PCR amplicons that demonstrated the exact same opinion sequences and the existence of many of the same quasispecies variations. The observance that aspects of the quasispecies structure revealed by massively parallel sequencing may also be detected after PCR and Sanger sequencing shows that this method can be helpful for tiny or hard to analyse samples.A steady rise in wildfire event seriousness and seas
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