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Aerosolizing viral vectors to your lung area of huge animals can optimize anatomical distribution. Here, we explain a technique for aerosolization of viral vectors into the airways of newborn pigs. Quickly, a pig is anesthetized and intubated with an endotracheal tube, and a microsprayer is passed through the endotracheal tube. A fine mist will be sprayed into the distal trachea. Widespread and uniform distribution of transgene appearance is crucial for developing successf