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Objectives The aim of this study has been to characterize carbapenem-resistant Klebsiella pneumoniae isolate, and to determine the resistance mechanisms involved, clonal relationship between strains and clinical and demographic data of the infected patients. Methods Clinical and demographical data from patients were collected and statistically analyzed. Antimicrobial susceptibility testing was performed and resistance genes were detected both phenotypic and genotypically. Conjugation assays were performed to show horizontal transferabi