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Shortages of allogeneic blood supplies for rhesus (Rh)-negative patients who are scheduled for major cancer surgeries may cause delays in surgical procedure, resulting in a prolonged length of hospital stay (LOHS). This study investigated the relationship of acute normovolemic hemodilution (ANH) with LOHS in this patient population. Rh-negative patients who underwent major cancer surgeries between January 2015 and April 2020 were included in this retrospective study. The primary outcome was LOHS. The secondary outcomes were length of p