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The phloem-restricted, insect-transmitted bacterium 'Candidatus Liberibacter asiaticus' (CLas) is associated with Huanglongbing (HL, the most devastating disease of citrus worldwide. The inability to culture CLas impairs the understanding of its virulence mechanisms and the development of effective management strategies to control this incurable disease. Previously, our research group used commercial grapefruit juice (GJ) to prolong the viability of CLas in vitro. In the present study, GJ was amended with a wide range of compounds and i