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001), than the controls. At the mRNA level, cytokines appeared partially induced. Double immunofluorescence labeling confirmed the costaining of these factors with inflammatory and glial markers. Conclusions The expression of hypoxia-related and inflammatory mediators was demonstrated for the first time in LSL specimens. These factors might play a role in multifactorial secondary lesion cascades underlying further damage to the neural placode in closed dysraphism.Background Cortical bone trajectories (CBTs) for pedicle screw inser