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The knowledge presented here can be employed for developing biomarkers for diagnostic and risk stratification purposes. This study examined whether a sleep enhancement protocol (SEP) could reduce nighttime room entries (NREs) for patients with orthopedic injury (OI) or acquired brain injury (ABI) in an inpatient rehabilitation facility. A two-wave prospective study assessing standard of care (SOC) versus SEP. Sixty-five participants completed baseline and follow-up questionnaires and wore an actigraph for approximately 7 days. In the SEP