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The concept is easily applied and does not require any system modifications. It is therefore well suited for open access applications where more instrument intensive approaches, such as "At-Column Dilution", would be less desirable. We will also show that the generic concept which we have titled "In-Column Dilution" can easily be applied to increase the detection sensitivity for analytical application as well by allowing injection of larger sample volumes without peak deterioration for purifications. V.The aim of our work was to devel