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A high-quality hole, without spatters around the periphery of hole entrance and without recast layer on the side-wall surface, in Ni-based superalloy coated with ceramic TBCs has been drilled. This research has potential applications to blade film cooling holes.Enzymes are biological catalysts that are comprised of small-molecule, metal, or cluster catalysts augmented by biopolymeric scaffolds. It is conceivable that early in chemical evolution, ancestral enzymes opted for simpler, easier to assemble scaffolds. Herein, we describe such