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Nonmydriatic montage widefield imaging acquisition time was significantly shorter than that of dilated clinical examination (p = 0.01. CONCLUSION Nonmydriatic montage widefield images were compared favorably with dilated fundus examination in defining DR severity; however, they are acquired more rapidly.INTRODUCTION Consensus in sample preparation for untargeted human fecal metabolomics is lacking. OBJECTIVES To obtain sample preparation with broad metabolite coverage for high-throughput LC-MS. METHODS Extraction solvent, solvent ratio a