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Within the split process, ultrafiltration molecular body weight cut off (MWCO), ultrasonic energy and polysorbate 80 concentraion had been selected as factors to modify the split performance. The ultrasonic regularity and energy were supplied by KQ-700DE ultrasonic system, based on the data analysis by response area methodology (RSM), the perfect UAU parameters had been as follows ultrafiltration MWCO of 50,000, ultrasonic power of 900 W and polysorbate 80 concentration of 15.00