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The recombinant RESOD exhibited maximal activity and stability with a temperature number of 0 °C to 10 °C. Additionally the ideal pH for the activity and stability was about 10. Nevertheless, RESOD had been responsive to some steel ions, particularly calcium. Additionally, the biological purpose of RESOD ended up being investigated in HeLa cells. It absolutely was unearthed that RESOD could lower the level of oxidation, and decrease the apoptosis lead from extortionate oxidant challenge.