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Here, we introduce an innovative "poison arrowhead" approach for disinfection based on a nanosheet bacterial inactivation system that acts synergistically to achieve sterilization rates of 99.99 % (Escherichia coli) over an ultrashort time period (≈0.5 min). The two-dimensional MoS2 "arrowhead" configuration has a sharp edge structure that enables the vigorous extraction of lipids from cell membranes and subsequent membrane disruptions. In the presence of permonosulfate, a strong oxidant, sulfur vacancies containing MoS2 activate the