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Addition of hydrochloric acid to the wastewater generated the precipitation of detergent and part of non-P3HB mobile mass. The ensuing precipitate was utilized as a carbon supply in biomass production and increased substrate-to-P3HB conversion.The role of EPS in removal of carbonaceous organic matters and NH4+-N in simulated mariculture wastewater was analyzed at salinity of 0-3.5% in a multi-soil-layering bioreactor. Results indicated that at 3.5% of