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P less then 0.05 was considered as significantly associated. Result The study revealed that, out of 200 participants, 96 (48%) have poor knowledge, whereas 104 (52%) showed good knowledge about HBV. Regarding the practice of participants, 79 (39.5%) of the students have good practice to prevent HBV, whereas 121 (59.5%) had poor practice towards HBV infection prevention. Conclusion Based on the current study, greater than half of the students who participated in the study have good knowledge of hepatitis B infection prevention and most of