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We discovered that high viral titers when you look at the midgut aren't s a delayed replication rate in mosquitoes, which lengthened the extrinsic incubation period (EIP) and decreased fitness relative to the wild-type virus. Because of this, the mutant virus displayed a diminished capability to get across anatomical obstacles throughout the infection pattern in mosquitoes, thus reducing the virus transmission rate. Our results reveal how discerning

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