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Right here, we purified a homologous helicase (Irc3op) associated with the thermotolerant fungus Ogataea polymorpha that keeps architectural integrity and catalytic activity at conditions above 40 °C. Irc3op can complement the breathing deficiency phenotype of a S. cerevisiae irc3Δ mutant, indicating conservation of biochemical functions. The ATPase task of Irc3op is best stimulated by branched and double- stranded DNA cofactors. Single-stranded DNA binds Irc3op firmly it is a wea
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