https://www.selleckchem.com/pr....oducts/z-yvad-fmk.ht
Integrating structural information with site-directed mutagenesis allowed us to identify features unique to each enzyme and provide mechanistic insight. In the epimerases, mutagenesis of H67, D173, N121, Y134 and Y132 suggested the presence of alternative catalytic residues. We showed that the reductases could reduce GDP-4-keto-6-deoxy-mannulose without prior epimerization though DdahC preferred the pre-epimerized substrate, and identified T110 and H180 as important for substrate specificity and catalytic efficacy. This information c
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