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A diafiltration process was then developed using a Biomax® 300 kDa polyethersulfone membrane to selectively remove excess guide RNA from a solution containing Cas9-bound guide RNA and free guide RNA. These results demonstrate the potential of using ultrafiltration for the removal of excess RNA during the production of functional ribonucleoprotein complexes. To estimate the implant surface temperature at titanium dental implants during calibrated irradiation using double wavelength laser. A double wavelength laser, 2780 nm Er,CrYSGG and

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