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Artificial LED source provides the possibility to regulate the lighting environment in plant factorys that use limited space to plant, aiming at high throughput and good quality. However, different parameters of light intensity, quality, and photoperiod will influence the growth and accumulation of bio-compounds in plants. In order to find the optimal setting of LED light for spinach planting, four group experiments were designed using the orthogonal testing method. According to the experimental results, for growth indexes including fresh