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05), whereas semen volume, subjective motility, and total sperm output were not affected (P 0.05). In frozen-thawed semen, total and progressive motility, viability, and straight-line velocity were lower in high THI compared to the low THI group (P less then 0.05), while H2O2 concentration, aberrant chromatin condensation, and abnormal spermatozoa were higher in the high THI group (P less then 0.05). Blastocyst rates were significantly higher when low THI samples were used (P less then 0.05). Moreover, the total cell number and trophecto

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