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The aim of this work was to evaluate the results of light quality using light-emitting diodes (LEDs) within the growth of L. filifolia grown in vitro after 45 times of culture, analyzing its volatile organic substances (VOCs), biochemical, and biometric qualities. This research had four treatments in accordance with the wavelength of LED lamps (i) white (control), (ii) blue, (iii) red, and (iv) a mixture of red + blue (mix). The light quality affected the growth, metabolism, and VO