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Here, we created a robust technique that, by combining an optimized comparison treatment with microCT imaging associated with the small adult mouse ovary, allowed 3D mapping and counting of follicles, from pre-antral secondary T4 (53.2 ± 12.7 μm in diameter) to antral T8 (321.0 ± 21.3 μm) and corpora lutea, alongside the major vasculature branches. Primordial and primary follicles (T1-T3) could never be observed. Our process highlighted, with unprecedent details, the key useful compartments regarding

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