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Our results demonstrated that 5 μM SFN improved all parameters of sperm including viability (P less then 0.001), motility, and morphology (P less then 0.05) after the freeze-thaw process. Furthermore, SFN reduced the levels of intracellular hydrogen peroxide (P less then 0.01) and superoxide anion (P less then 0.05). Also, SFN significantly increased the percentage of viable sperm cells with the intact plasma membrane (P less then 0.001) and decreased the level of lipid peroxidation after the freeze-thaw process (P less then 0.01).Our fi